ABSTRACT
BACKGROUND: After tissue-engineering products transplantation, angiogenesis played an important role in the function restoring of defective organs. The natural tissue-engineering materials had a wide application in tissue engineering due to its favorable biocompatibility and degradability, at the same time its pro-angiogenic function enhanced the achievement ratio of tissue-engineering products transplantation. Therefore, they attract much attention during recent years. OBJECTIVE: To summarize the research status of incubating induced angiogenesis of tissue-engineered natural scaffold, so as to give some theoretical basis for further study on clinical application of natural tissue engineering materials. METHODS: Relevant literatures in PubMed and Springerlink published between January1995 and June 2009 were searched by compute with the key words of "tissue-engineering products, natural materials" in English. While relevant Chinese articles in CKNI published between January1999 and June 2007 were also searched with the key words of "tissue-engineering natural materials, collagen, chitosan, fibrin" in Chinese. After primary selection, inclusive articles were those about study and experimental study of induced angiogenesis of tissue-engineered natural scaffold. Exclusive criteria: repetitive and obsolescent articles. A total 35 literatures were finally analyzed in accordance with the criteria. RESULTS AND CONCLUSION: The natural tissue engineering materials were synthesized by macromolecules out of normal tissue, whose multiple bioinformation provided signal for cells and benefited for cellular adhesion and maintenance. Collagen protein, fiber gel protein, and chitosan summarized in this study were beneficial for inducing angiogenesis but limited to mechanical characteristics. Therefore, to construct natural materials inducing angiogenesisis is prospect.
ABSTRACT
Some different membranes were prepared by Chitosan with the degree of deacetylation (DD) of 63.7%, 73.7%, 83% and 97% respectively. To study the biocompatibility of Chitosan membrane toward corneal stromal cells, the rabbit cells were cultured on the surface of different DD chitosan membranes. The morphological characteristics, the cell-adhesion, the cell proliferation and the activity of LDH in the medium were investigated. The results of experiment shows that the DD of Chitosan has very significant effect on the biocompatibility of Chitosan membrane toward corneal stromal cells. The more DD of Chitosan, the less injury was made to corneal stromal cells by the chitosan membrane, which is favor of the growing and adhesion of corneal stromal cells. The biocompatibility of the membrane made with low DD Chitosan with corneal stromal cells became worse.